Simultaneous estimation of amlodipine besylate and nebivolol hydrochloride in combined dosage form by RP-HPLC

A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the simultaneous estimation of Amlodipine Besylate and Nebivolol hydrochloride in pharmaceutical dosage forms. The method was carried out on a Luna C-18, 5 μ column with a mobile phase consisting of 0.005M ammonium acetate solution, acetonitrile and triethylamine in the ratio 60:40:0.1 (v/v) and pH 3.0 was adjusted with orthophosphoric acid. Detection was carried out at 269nm at a flow rate of 1.5 ml/min. The retention time of Amlodipine and Nebivolol was 3.911 and 5.818 min, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection and limit of quantitation. The proposed method can be used for the estimation of both the components in simultaneously in pharmaceutical tablet formulation. The method was found to be linear in the range of 10-30 μg/ml for both Amlodipine and Nebivolol. In the linearity study, regression equation and coefficient of correlation for Amlodipine and Nebivolol were found to be y = 59687x + 56654, r= 0.9923 and y = 32052x + 37541, r= 0.9992, respectively.